Journal: Hereditas
Article Title: PTTG1 regulates the TGF-β/Smad pathway to promote the growth and metastasis of gastric cancer cells
doi: 10.1186/s41065-026-00647-0
Figure Lengend Snippet: Silencing PTTG1 suppresses epithelial-mesenchymal transition in gastric cancer cells. Western blot analysis of EMT-associated markers (E-cadherin, N-cadherin, α-SMA, and Vimentin) in SGC-7901 and HGC-27 cells transfected with control, si-NC (scramble), or si-PTTG1. GAPDH served as the internal loading control. Densitometric quantification of protein expression relative to GAPDH is shown in bar graphs. Each experiment was repeated three times. Data are presented as mean ± SD. *** P < 0.001, ** P < 0.01 versus si-NC
Article Snippet: Human gastric cancer cell lines, including AGS and MKN-45, and normal gastric epithelial cells (GES-1), were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).
Techniques: Western Blot, Transfection, Control, Expressing